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. 1984 Sep;53(1):79–86.

Inhibition of autologous mixed lymphocyte reaction by monoclonal antibodies specific for the β chain of HLA-DR antigens

M Kasahara, H Ikeda, K Ogasawara, N Ishikawa, T Okuyama, Y Fukasawa, H Kojima, H Kunikane, S Hawkin, T Ohhashi, T Natori, A Wakisaka, Y Kikuchi, M Aizawa
PMCID: PMC1454738  PMID: 6205985

Abstract

Recent studies using rabbit antisera to the separated HLA-DR α and β subunits have suggested that α chain-specific, but not β chain-specific, antisera inhibit T cell proliferative responses in primary and secondary human autologous mixed lymphocyte reaction (AMLR).

In the present study, with the aid of sequential co-precipitation assays and Western blotting methods, a monoclonal rat alloantibody 1E4, specific for the β chain of rat class II molecules carrying an Ia determinant Ba-2.7, was characterized to recognize a monomorphic determinant located on the β chain of DR antigens. This antibody and a murine monoclonal antibody HU-4, also specific for the β chain of DR antigens, strongly inhibited both primary and secondary AMLR through a mechanism distinct from an antibody-dependent cell-mediated cytotoxicity reaction. These results indicate that the inhibition of AMLR is not a unique feature of DR α-specific antibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. De Kretser T. A., Crumpton M. J., Bodmer J. G., Bodmer W. F. Two-dimensional gel analysis of the polypeptides precipitated by a polymorphic HLA-DR1,2,w6 monoclonal antibody: evidence for a third locus. Eur J Immunol. 1982 Jul;12(7):600–606. doi: 10.1002/eji.1830120713. [DOI] [PubMed] [Google Scholar]
  2. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gottlieb A. B., Fu S. M., Yu D. T., Wang C. Y., Halper J. P., Kunkel H. G. The nature of the stimulatory cell in human allogeneic and autologous MLC reactions; role of isolated IgM-bearing B cells. J Immunol. 1979 Oct;123(4):1497–1503. [PubMed] [Google Scholar]
  4. Huber C., Fink U., Leibold W., Schmalzl F., Peterson P. A., Klareskog L., Braunsteiner H. The role of adherent HLA-DR+ mononuclear cells in autologous and allogeneic MLR. J Immunol. 1981 Aug;127(2):726–731. [PubMed] [Google Scholar]
  5. Johnson J. P., Meo T., Riethmüller G., Schendel D. J., Wank R. Direct demonstration of an HLA-DR allotypic determinant on the low molecular weight (beta) subunit using a mouse monoclonal antibody specific for DR3. J Exp Med. 1982 Jul 1;156(1):104–111. doi: 10.1084/jem.156.1.104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kasahara M., Ogasawara K., Ikeda H., Okuyama T., Ishikawa N., Takenouchi T., Wakisaka A., Kikuchi Y., Aizawa M. A monoclonal antibody that detects a polymorphic determinant common to HLA-DR1 and 2. Tissue Antigens. 1983 Feb;21(2):105–113. doi: 10.1111/j.1399-0039.1983.tb00378.x. [DOI] [PubMed] [Google Scholar]
  7. Koide Y., Awashima F., Yoshida T. O., Takenouchi T., Wakisaka A., Moriuchi J., Aizawa M. The role of three distinct Ia-like antigen molecules in human T cell proliferative responses: effect of monoclonal anti-Ia-like antibodies. J Immunol. 1982 Sep;129(3):1061–1069. [PubMed] [Google Scholar]
  8. Kuntz M. M., Innes J. B., Weksler M. E. Lymphocyte transformation induced by autologous cells. IV. Human T-lymphocyte proliferation induced by autologous or allogeneic non-T lymphocytes. J Exp Med. 1976 May 1;143(5):1042–1054. doi: 10.1084/jem.143.5.1042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mingari M. C., Moretta L. Role of Ia antigens in the human autologous mixed lymphocyte reaction. Eur J Immunol. 1982 Jan;12(1):98–100. doi: 10.1002/eji.1830120119. [DOI] [PubMed] [Google Scholar]
  10. NISONOFF A., WISSLER F. C., WOERNLEY D. L. Properties of univalent fragments of rabbit antibody isolated by specific adsorption. Arch Biochem Biophys. 1960 Jun;88:241–249. doi: 10.1016/0003-9861(60)90229-0. [DOI] [PubMed] [Google Scholar]
  11. Natori T., Ohhashi T., Inomata T., Fujimoto Y., Ishida Y., Kasahara M., Aizawa M. Immunochemical evidence for multiple beta units of the class II molecule in the rat. J Immunogenet. 1983 Dec;10(6):439–452. doi: 10.1111/j.1744-313x.1983.tb01031.x. [DOI] [PubMed] [Google Scholar]
  12. Ohhashi T., Iwabuchi K., Natori T., Nakagawa H., Kikuchi Y., Aizawa M. The B region-associated antigens and MLR phenotypes in the Japanese inbred strains of rats. J Immunogenet. 1981 Jun;8(3):191–206. doi: 10.1111/j.1744-313x.1981.tb00757.x. [DOI] [PubMed] [Google Scholar]
  13. Opelz G., Kiuchi M., Takasugi M., Terasaki P. I. Autologous stimulation of human lymphocyte subpopulation. J Exp Med. 1975 Nov 1;142(5):1327–1333. doi: 10.1084/jem.142.5.1327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Palacios R., Claesson L., Möller G., Peterson P. A., Möller E. The alpha chain, not the beta chain of HLA-DR antigens participates in activation of T cells in autologous mixed lymphocyte reaction. Immunogenetics. 1982;15(4):341–356. doi: 10.1007/BF00364258. [DOI] [PubMed] [Google Scholar]
  15. Palacios R., Möller G. HLA-DR antigens render resting T cells sensitive to interleukin-2 and induce production of the growth factor in the autologous mixed lymphocyte reaction. Cell Immunol. 1981 Sep 1;63(1):143–153. doi: 10.1016/0008-8749(81)90035-6. [DOI] [PubMed] [Google Scholar]
  16. Shackelford D. A., Strominger J. L. Demonstration of structural polymorphism among HLA-DR light chains by two-dimensional gel electrophoresis. J Exp Med. 1980 Jan 1;151(1):144–165. doi: 10.1084/jem.151.1.144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tomonari K. Cytotoxic T cells generated in the autologous mixed lymphocyte reaction. I. Primary autologous mixed lymphocyte reaction. J Immunol. 1980 Mar;124(3):1111–1121. [PubMed] [Google Scholar]
  18. Tomonari K., Wakisaka A., Aizawa M. Self recognition by autologous mixed lymphocyte reaction-primed cells. J Immunol. 1980 Oct;125(4):1596–1600. [PubMed] [Google Scholar]
  19. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Weksler M. E., Kozak R. Lymphocyte transformation induced by autologous cells. V. Generation of immunologic memory and specificity during the autologous mixed lymphocyte reaction. J Exp Med. 1977 Dec 1;146(6):1833–1838. doi: 10.1084/jem.146.6.1833. [DOI] [PMC free article] [PubMed] [Google Scholar]

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