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Effect of NADPH on NADH-dependent 7-ethoxyresorufin metabolism. NADPH- and NADH-dependent EROD activities were measured with CYP1A2 (5 pmol) in E. coli membranes coexpressing either CPR or the mutants W676H and W676A, as described in the legend to Table 1. Reactions were started with NADH (500 μM). After 5 min, NADPH (50 μM) was added, and the reaction recorded for additional 5 min (CPR, circle; W676A, squares; W676H, asterix).
