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| IPP isomerase | Km (µM) | Vmax (µmol/h/mg) | Vmax/Km | Relative activity (%) |
|---|---|---|---|---|
| Wild type | 3.5 ± 0.2 | 2.1 ± 0.05 | 0.6 | 100 |
| R51K | 18.5 ± 2.0 | 0.09 ± 0.007 | 0.005 | 4.2 |
| K55R | 15.0 ± 3.6 | 0.58 ± 0.1 | 0.04 | 28 |
| K55A | 14.0 ± 1.9 | 1.386 ± 0.04 | 0.099 | 66 |
| C67A | – | – | – | 0 |
| R83K | 3.0 ± 0.5 | 2.2 ± 0.15 | 0.93 | 104 |
| E87Q | 11.5 ± 0.1 | 0.0014 ± 1 × 10–5 | 1.22 × 10–4 | 0.07 |
| E116Q | 21.0 ± 0.5 | 0.0018 ± 2 × 10–5 | 8.81 × 10–4 | 0.09 |
| W161F | 0.8 ± 0.75 | 0.017 ± 0.01 | 0.021 | 0.8 |
