Fig. 2. DsbC G49R is a monomer. The DsbC mutant G49R was purified and analyzed by analytical gel filtration. DsbC G49R shows a clear difference in migration on a Sephadex 200 column as compared with wild-type DsbC. The inset shows a standard curve generated with proteins of known molecular weight (open circles): bovine serum albumin (66 kDa), carbonic anhydrase (29 kDa), cytochrome c (12.4 kDa) and apoprotinin (6.5 kDa). The apparent molecular weights of wild-type DsbC and DsbC G49R (filled triangles) were determined according to this curve. An apparent mol. wt of 31.6 kDa was calculated for DsbC G49R. Wild-type DsbC runs with an apparent mol. wt of 51.4 kDa. This suggests that G49R is monomeric.