Table I. Effect of dsbD depletion on motility and alkaline phosphatase activity.
Strain | Relevant genotype | DsbC variant expressed | Motility | Alkaline phosphatase activity (% of wt) |
---|---|---|---|---|
MC1000 | wt | – | + + + | 100 |
FED126 | dsbD– | – | + + + | 109 |
MB102 | dsbA– dsbD– | – | – | 10 |
MB102 pMB69 | dsbA– dsbD– | wt | – | 16 |
MB102 pMB89 | dsbA– dsbD– | G49R | + + | 76 |
MB102 pMB90 | dsbA– dsbD– | G49E | + + | 88 |
MB102 pMB91 | dsbA– dsbD– | M27K | + + | 82 |
MB102 pMB92 | dsbA– dsbD– | I47K | + + | 74 |
MB102 pMB93 | dsbA– dsbD– | V54K | + + | 73 |
MB102 pMB94 | dsbA– dsbD– | H45D | + + | 75 |
MB102 pMB96 | dsbA– dsbD– | Δ1–76 | + + | 44 |