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Fluorescence images of V2R-GFP and V2R(R137H)-GFP in HEK-293 cells. Cells expressing V2R-GFP (Upper) or V2R(R137H)-GFP (Lower) were treated with vehicle or AVP for 30 min at 37°C. The agonist-mediated redistribution of wild-type receptor (Upper) from the plasma membrane to endocytic vesicles contrasts with the agonist-independent localization of the V2R(R137H)-GFP in endosomes (Lower). Bar = 25 μM.
