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. 2000 Dec 26;98(1):93–98. doi: 10.1073/pnas.011303698

Figure 3.

Figure 3

Fluorescence images of the association between βarrestin2-GFP and V2R or V2R(R137H) in HEK-293 cells. Left shows the agonist-independent distribution of βarrestin2-GFP in living cells expressing the V2R (Upper) or V2R(R137H) (Lower). Without agonist treatment, βarrestin2-GFP is cytosolic in cells containing wild-type V2R (Left Upper), but in cells containing the V2R(R137H) (Left Lower) it is also in endocytic vesicles. Following 30 min of exposure to 100 nM AVP (Right), βarrestin-GFP is localized on endocytic vesicles with both receptor subtypes.