Fig. 2. Effects of LAP2-c mutants on nuclear assembly in Xenopus egg extracts. Nuclear assembly reactions were supplemented at time zero with no additions (Control), wild-type LAP2-c protein or each LAP2-c mutant (numbered as shown in Figure 1) to a final concentration of 10 µM. Nuclei were imaged by phase contrast microscopy after 3–5 h, to allow nuclei to reach their terminal arrest phenotype; images shown are typical of at least 20 nuclei examined. The nuclear envelope is visible as a thin continuous dark line (arrow in Control). When nuclear assembly is arrested by LAP2-c, the surface area is smaller and the thin dark line is discontinuous and concave (‘scalloped’; arrow in LAP2-c; see Gant et al., 1999). Bar = 20 µm.