Skip to main content
. 2000 Dec 26;98(1):99–104. doi: 10.1073/pnas.021537498

Figure 3.

Figure 3

Demonstration of the covalent loading of the HMWP1 fragments with L-[35S]cysteine catalyzed by HMWP2 1–1491. (A) Coomassie-stained gel of TCA-precipitated reaction mixtures. (B) Autoradiograph of the gel. All reaction mixtures contained one holo-HMWP1 fragment (1 μM), HMWP1 1–1491 (0.15 μM), and [35S]cysteine (100 μM, 285 Ci/mol). ATP was omitted in the odd-numbered lanes and added (2 mM) in the even-numbered lanes except lane 0. Lane 0, molecular weight markers; lanes 1 and 2, holo-1–1895; lanes 3 and 4, holo-1812–3163; lanes 5 and 6, holo-1896–3163.