Table 1.
Kinetic parameters of phosphopantetheinylation of apo forms of the 1-1895 (ACP), 1896-3163 (PCP3), and 1812-3163 (ACP and PCP3) fragments by three different PPTases at 37°C
| Substrate | Enzyme | kcat, min−1 | KM, μM | kcat/KM (μM−1min−1) |
|---|---|---|---|---|
| 1-1895 | Sfp | 0.69 ± 0.03 | 0.026 ± 0.009 | 26.5 |
| ACPS | 1.6 ± 0.2 | 0.10 ± 0.04 | 16.0 | |
| EntD | 0.22 ± 0.07 | 1.9 ± 0.9 | 0.12 | |
| 1896-3163 | Sfp | 1.3 ± 0.2 | 0.6 ± 0.2 | 2.0 |
| EntD | 0.11 ± 0.01 | 0.46 ± 0.15 | 0.24 | |
| ACPS | No measurable phosphopantetheinylation | |||
| 1812-3163 | Sfp | 9.0 ± 0.5 | 1.1 ± 0.2 | 8.4 |
| ACPS | 12.4 ± 2.3 | 4.9 ± 1.5 | 2.5 | |
| EntD | 8.4 ± 1.3 | 3.1 ± 0.9 | 2.7 | |