Table 3.
Kinetic parameters of cysteinylation of holo forms of the 1-1895 (ACP), PCP3-TE, and 1812-3163 (ACP and PCP3) fragments in trans by HMWP2 1-1491 at 37°C
Substrate | kcatmin−1 | KM, μM | kcat/KM, μM−1·min−1 |
---|---|---|---|
1812-3163 | 33 ± 1 | 10 ± 1 | 3.3 |
PCP3-TE | 61 ± 13 | 40 ± 15 | 1.5 |
1-1895 | No measurable cysteinylation |