Table I. Effects of cpx mutations and NlpE overproduction on P pili-mediated HA titers.
| Genotype | PhenotypeStrain/plasmid | HA titer |
|---|---|---|
| MC4100/pPAP5a | wild type/P pili (natural promoter) | 64 |
| TR51/pPAP5a | CpxR null/P pili (natural promoter) | 64 |
| TR14/pPAP5a | Cpx*/P pili (natural promoter) | 256 |
| TR20/pPAP5a | Cpx*/P pili (natural promoter) | 256 |
| MC4100/pRHU845a | wild type/P pili | 64 |
| MC4100/pRHU845/pLD404a | wild type/P pili/NlpE | 128 |
| MC4100/pRHU845b | wild type/P pili | 64 |
| MC4100/pRHU845/pLD404b | wild type/P pili/NlpE | 128 |
| MC4100/pRHU845c | wild type/P pili | 0 |
| MC4100/pRHU845/pLD404c | wild type/P pili/NlpE | 8 |
| MC4100/pFJ29d | wild type/P pili | 32 |
| TR51/pFJ29d | CpxR null/P pili | 32 |
| TR14/pFJ29d | Cpx*/P pili | 64 |
| TR20/pFJ29d | Cpx*/P pili | 32 |
The HA titer was quantitated after 3 days passage of the strains on TSA platesa, after overnight growth in shaking Luria broth without glucoseb or with 0.5% glucose addedc, or after growth to mid-logarithmic phased.