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. 2001 Mar 15;20(6):1425–1438. doi: 10.1093/emboj/20.6.1425

Table II. Plasmids used in this study.

Plasmid Allele Reference
Plasmids encoding GST fusion proteins
 pHQ242 GST under ADH1 promoter in 2µ TRP1 plasmid pPM237 (L.Prakash) this study
 pHQ601 GST-GCN2(970–1497) in pHQ242 backbone this study
 pHQ529 GST-GCN2(720–999) in pGEX-5X-1 (Pharmacia Biotech.) this study
 pHQ531 GST-GCN2(1498–1659) in pGEX-5X-1 Qiu et al. (1998)
 pHQ551 GST-GCN2(568–998) in pGEX-5X-1 Qiu et al. (1998)
 pHQ701 GST-GCN2(1315–1497) in pGEX-5X-1 this study
 pHQ704 GST-GCN2(970–1156) in pGEX-5X-1 this study
 pHQ993 GST-GCN2(720–809) in pGEX-5X-1 this study
 pHQ994 GST-GCN2(720–779) in pGEX-5X-1 this study
 pHQ1015 GST-GCN2(970–1156)/Δ984–1016 in pGEX-5X-1 this study
 pHQ1016 GST-GCN2(970–1156)/Δ1048–1071 in pGEX-5X-1 this study
 pHQ1018 GST-GCN2(970–1156)/Δ1087–1106 in pGEX-5X-1 this study
 pHQ1019 GST-GCN2(970–1156)/Δ1107–1125 in pGEX-5X-1 this study
 pHQ1020 GST-GCN2(970–1156)/Δ1128–1149 in pGEX-5X-1 this study
 pHQ1025 GST-GCN2(970–1156)/Δ1018–1047 in pGEX-5X-1 this study
 pHQ1038 GST-GCN2(970–1156)/Δ1071–1087 in pGEX-5X-1 this study
 pHQ1059 GST-GCN2(970–1156)/as4 in pGEX-5X-1 this study
 pHQ1073 GST-GCN2(970–1156)/as3/4 in pGEX-5X-1 this study
Plasmids for in vitro translation of GCN2 segments
 pHQ541 GCN2(970–1497) under T7 promoter in pGEM-3Z (Promega) Qiu et al. (1998)
 pHQ596 GCN2(970–1315) in pGEM-3Z this study
 pHQ597 GCN2(970–1383) in pGEM-3Z this study
 pHQ599 GCN2(1086–1497) in pGEM-3Z this study
 pHQ664 GCN2(1315–1497) in pGEM-3Z this study
 pHQ665 GCN2(970–1156) in pGEM-3Z this study
 pHQ666 GCN2(970–1245) in pGEM-3Z this study
 pHQ667 GCN2(1028–1497) in pGEM-3Z this study
 pHQ668 GCN2(1130–1497) in pGEM-3Z this study
 pHQ669 GCN2(1193–1497) in pGEM-3Z this study
 pHQ670 GCN2(1255–1497) in pGEM-3Z this study
 pHQ671 GCN2(970–1120) in pGEM-3Z this study
 pHQ680 GCN2(970–1086) in pGEM-3Z this study
 pHQ683 GCN2(1353–1497) in pGEM-3Z this study
 pHQ1008 GCN2(970–1156)/Δ984–1016 in pGEM-3Z this study
 pHQ1009 GCN2(970–1156)/Δ1018–1047 in pGEM-3Z this study
 pHQ1010 GCN2(970–1156)/Δ1048–1071 in pGEM-3Z this study
 pHQ1012 GCN2(970–1156)/Δ1087–1106 in pGEM-3Z this study
 pHQ1013 GCN2(970–1156)/Δ1107–1125 in pGEM-3Z this study
 pHQ1014 GCN2(970–1156)/Δ1128–1149 in pGEM-3Z this study
 pHQ1024 GCN2(970–1497)/Δ1315–1388 in pGEM-3Z this study
 pHQ1030 GCN2(970–1497)/Δ1029–1119 in pGEM-3Z this study
 pHQ1037 GCN2(970–1156)/Δ1071–1087 in pGEM-3Z this study
 pHQ1058 GCN2(970–1156)/as4 in pGEM-3Z this study
 pHQ1066 GCN2(970–1156)/as3/4 in pGEM-3Z this study
Plasmids encoding GCN2 and derivatives
 p332 gcn2-m2 in p630 backbone Wek et al. (1995)
 p630 wild-type GCN2 in in YEp24 (Botstein et al., 1979) 2µ, URA3 Wek et al. (1990)
 p2461 gcn2-Δ1536–1659 in p630 backbone Qiu et al. (1998)
 pHQ644 GCN2, CEN6, ARSH4, URA3, a derivative of p722 (Wek et al., 1990) this study
 pHQ1039 gcn2-Δ1018–1047 in pHQ644 backbone this study
 pHQ1040 gcn2-Δ1107–1125 in pHQ644 backbone this study
 pHQ1041 gcn2-Δ984–1016 in pHQ644 backbone this study
 pHQ1042 gcn2-Δ1048–1071 in pHQ644 backbone this study
 pHQ1043 gcn2-Δ1087–1106 in pHQ644 backbone this study
 pHQ1044 gcn2-Δ1018–1047 in p630 backbone this study
 pHQ1045 gcn2-Δ1048–1071 in p630 backbone this study
 pHQ1047 gcn2-Δ1071–1087 in pHQ644 backbone this study
 pHQ1051 gcn2-Δ1128–1149 in pHQ644 backbone this study
 pHQ1076 gcn2-as4 in pHQ644 backbone this study
 pHQ1077 gcn2-as3/4 in pHQ644 backbone this study
 pHQ1081 gcn2-as4 in p630 backbone this study
 pHQ1082 gcn2-as3/4 in p630 backbone this study
 pHQ1092 gcn2-Δ1315–1383 in pHQ644 backbone this study
 pDH103 GCN2-FL in pEMBLyex4 (Cesareni and Murray, 1987) backbone Dong et al. (2000)
 pDH104 gcn2-FL-m2 in pEMBLyex4 backbone Dong et al. (2000)
 pHQ1048 gcn2-FL-Δ1018–1047 in pEMBLyex4 backbone this study
 pHQ1049 gcn2-FL-Δ1048–1071 in pEMBLyex4 backbone this study
 pHQ1084 gcn2-FL-as3/4 in pEMBLyex4 backbone this study
Plasmids for two-hybrid analysis of GCN2 segments
 p2247 LexA-HA in pEG202 (Golemis et al., 1996) backbone Zhang et al. (1997)
 pHQ311 LexA-GCN2(1498–1659) in pEG202 backbone Qiu et al. (1998)
 pHQ400 LexA-HA-GCN2(27–1659) in p2247 backbone Qiu et al. (1998)
 pHQ433 LexA-GCN2(720–999) in pEG202 backbone Qiu et al. (1998)
 pHQ588 LexA-HA-GCN2(970–1497) in p2247 backbone Qiu et al. (1998)
 pHQ689 LexA-GCN2(970–1156) in pEG202 backbone this study
 pHQ428 B42-GCN2(720–999) in pJG4-5 (Golemis et al., 1996) backbone Qiu et al. (1998)
 pHQ429 B42-GCN2(750–999) in pJG4-5 backbone Qiu et al. (1998)
 pHQ468 B42-GCN2(780–999) in pJG4-5 backbone this study
 pHQ469 B42-GCN2(810–999) in pJG4-5 backbone this study
 pHQ470 B42-GCN2(740–999) in pJG4-5 backbone this study
 pHQ471 B42-GCN2(720–929) in pJG4-5 backbone this study
 pHQ472 B42-GCN2(720–959) in pJG4-5 backbone this study
 pHQ491 B42-GCN2(720–869) in pJG4-5 backbone this study
 pHQ492 B42-GCN2(720–899) in pJG4-5 backbone this study
 pHQ823 B42-GCN2(720–999)/E803V in pJG4-5 backbone this study
 pHQ841 B42-GCN2(720–839) in pJG4-5 backbone this study
 pHQ842 B42-GCN2(720–809) in pJG4-5 backbone this study
 pHQ843 B42-GCN2(720–779) in pJG4-5 backbone this study