Fig. 5.

Kinetic of release of infectivity from Huh7–Lunet cells (Fig. 4) after transfection with HCV genomes specified to the right. Four-, 24-, 48-, 72-, and 96-h posttransfection supernatants were harvested and used for TCID₅₀ determinations. For clarity, each pair of chimeric genomes is shown in comparison with JFH1 WT and the JFH1ΔE1E2 mutant. Note the different kinetics and efficiencies of release of infectivity achieved with the various chimeras. A summary of peak titers of infectivity is given in Table 1.