TABLE 2.

Mutants designed with Rosetta

Position	30	36	38	39	64	68	75	77	mAb bindinga	αLI domain tetramerbindinga	Predicted binding energy (relative to WT)
									% of WT		kcal/mol
WT	L	P	P	K	M	N	T	K	100	100	0
1		K							75 ± 10	75 ± 9	31.0
2		Q							50 ± 12	35 ± 6	31.4
3				R					50 ± 15	50 ± 15	31.6
4					F				105 ± 8	138 ± 12	30.5
5					Y				90 ± 6	135 ± 10	30.5
6						E			100 ± 5	120 ± 8	31.5
7	Y								86 ± 12	30 ± 16	31.2
8			W						55 ± 9	110 ± 15	30.6
9			R						150 ± 10	210 ± 30	31.3
10								F	95 ± 6	120 ± 6	30.5
11								Y	90 ± 7	115 ± 8	30.5
12							I		92 ± 7	187 ± 20	30.7
13	Y	E	W	R	F		I	F	80 ± 12	10 ± 10	33.4

^aSee Table 1 for details.