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CD19+ isolated IgM+/IgG- peripheral B cells were analyzed for surface expression of CD20 and IgG (upper panel). IgG, IgM, and IgD expression was determined following subsequent negative selection using anti-IgG antibody (lower panel). Percentages of positively stained cells are indicated in the right corner of each histogram. The stippled line represents the isotype control for each antibody.
