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p15Ink4B mRNA expression increases in response to TGFβ1. Primary OC cells (OC1 & OC2) were untreated (UT) or treated (T) with 4 pM TGFβ1. A) Northern blot to detect p15Ink4B using cDNA probes produced poor quality signal with background, making results difficult to interpret. B) The same blot was re-probed using radiolabeled p15Ink4B cRNA which clearly improved results, providing a high quality signal and easily interpretable results. GAPDH mRNA is used as a loading control.
