Figure 5.

Hormone stimulation of MCF-7 cells. Cells were grown in phenol-red free media without serum for 36 h before selective stimulation with 10 nM β-estradiol (E2) or 10 nM progesterone (Pg), or no stimulation (controls). Cells grown in media containing 10% FCS were used as controls (with FCS). Following stimulation, cellular RNAs were harvested and analyzed for endogenous BRCA1 and Id4 expression levels by using quantitative real-time RT-PCR. Numbers are given as percent (mean ± SEM) in comparison to cells grown without serum.