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. 1970 Jul;19(1):169–179.

Allotypic suppression of adult mouse spleen cells

Hilary R Anderson
PMCID: PMC1455615  PMID: 4922028

Abstract

Adult mouse spleen cells were transferred into irradiated adult mice with xenogeneic erythrocytes. Antibody-producing cells were measured by the localized haemolysis in gel (LHG) assay. Anti-allotype serum directed against the γG2a allotype of the donor cells, given to the recipients at the same time as antigen and spleen cells, suppressed the plaque-forming cells (PFC) producing antibody of that allotype, and had little effect on the other classes investigated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DRAY S. Effect of maternal isoantibodies on the quantitative expression of two allelic genes controlling gamma-globulin allotypic specificities. Nature. 1962 Aug 18;195:677–680. doi: 10.1038/195677a0. [DOI] [PubMed] [Google Scholar]
  2. David G. S., Todd C. W. Suppression of heavy and light chain allotypic expression in homozygous rabbits through embryo transfer. Proc Natl Acad Sci U S A. 1969 Mar;62(3):860–866. doi: 10.1073/pnas.62.3.860. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dubiski S., Fradette K. The feed-back mechanism in immunoglobulin synthesis. Proc Soc Exp Biol Med. 1966 May;122(1):126–130. doi: 10.3181/00379727-122-31070. [DOI] [PubMed] [Google Scholar]
  4. Dubiski S. Suppression of synthesis of allotypically defined immunoglobulins and compensation by another sub-class of immunoglobulin. Nature. 1967 Jun 24;214(5095):1365–1366. doi: 10.1038/2141365a0. [DOI] [PubMed] [Google Scholar]
  5. HERZENBERG L. A. A CHROMOSOME REGION FOR GAMMA 2A AND BETA 2A GLOBULIN H CHAIN ISOANTIGENS IN THE MOUSE. Cold Spring Harb Symp Quant Biol. 1964;29:455–462. doi: 10.1101/sqb.1964.029.01.048. [DOI] [PubMed] [Google Scholar]
  6. Herzenberg L. A., Goodlin R. C., Rivera E. C. Immunoglobulin synthesis in mice: suppression by anti-allotype antibody. J Exp Med. 1967 Oct 1;126(4):701–713. doi: 10.1084/jem.126.4.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Klein J., Herzenberg L. A. Congenic mouse strains with different immunoglobulin allotypes. I. Breeding scheme, histocompatibility tests, and kinetics of gamma G2a-globulin production by transferred cells for C3H.SW and its congenic partner CWB/5. Transplantation. 1967 Nov;5(6):1484–1495. doi: 10.1097/00007890-196711000-00013. [DOI] [PubMed] [Google Scholar]
  8. Lance E. M., Ford P. J., Ruszkiewicz M. The use of subcellular fractions to raise anti-lymphocytic serum. Immunology. 1968 Oct;15(4):571–580. [PMC free article] [PubMed] [Google Scholar]
  9. Mage R., Dray S. Persistent altered phenotypic expression of allelic gamma-G-immunoglobulin allotypes in heterozygous rabbits exposed to isoantibodies in fetal and neonatal life. J Immunol. 1965 Sep;95(3):525–535. [PubMed] [Google Scholar]
  10. Mage R., Young G. O., Dray S. An effect upon the regulation of gene expression: allotype suppression at the a locus in heterozygous offspring of immunized rabbits. J Immunol. 1967 Mar;98(3):502–509. [PubMed] [Google Scholar]
  11. Sell S. Studies on rabbit lymphocytes in vitro. 8. The relationship between heterozygosity and homozygosity of lymphocyte donor and per cent blast transformation induced by antiallotype sera. J Exp Med. 1968 Jun 1;127(6):1139–1148. doi: 10.1084/jem.127.6.1139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sell S. Studies on rabbit lymphocytes in vitro. IX. The suppression of antiallotype-induced blast transformation in lymphocyte cultures from allotypically suppressed donors. J Exp Med. 1968 Aug 1;128(2):341–355. doi: 10.1084/jem.128.2.341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Warner N. L., Herzenberg L. A., Goldstein G. Immunoglobulin isoantigens (allotypes) in the mouse. II. Allotypic analysis of three gammaG2-myeloma proteins from (NZB x BALB/c)F1 hybrids and of normal gammaG2-globulins. J Exp Med. 1966 Apr 1;123(4):707–721. doi: 10.1084/jem.123.4.707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wortis H. H., Dresser D. W., Anderson H. R. Antibody production studied by means of the localized haemolysis in gel (LHG) assay. 3. Mouse cells producing five different classes of antibody. Immunology. 1969 Jul;17(1):93–110. [PMC free article] [PubMed] [Google Scholar]
  15. Wortis H. H., Taylor R. B., Dresser D. W. Antibody production studied by means of the LHG assay. I. The splenic response of CBA mice to sheep erythrocytes. Immunology. 1966 Dec;11(6):603–616. [PMC free article] [PubMed] [Google Scholar]
  16. Wortis H. H., Taylor R. B., Dresser D. W. Antibody production studied by means of the localized haemolysis in gel (LHG) assay. II. Assay procedure. Immunology. 1968 Jan;14(1):69–79. [PMC free article] [PubMed] [Google Scholar]

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