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. 2002 Dec 9;4:94–104. doi: 10.1251/bpo39

Fig. 2.

Fig. 2

MALDI-TOF mass spectrometry identification of Veli3 as a binding partner of the PDZ ligand of the 5-HT2C receptor. A. Areas of interest of silver-stained 2-D gels obtained in experiments using the GST902CSSV, GST902CSSA and GST baits, respectively, and showing the specific recruitment of one protein spot by the PDZ ligand of the 5-HT2C receptor. B. MALDI-TOF peptide mass map obtained from in-gel trypsin digestion of this spot. Ion signals with measured masses that matched calculated masses of protonated tryptic peptides of mouse Veli3 are indicated. T indicates the ion signals corresponding to the autolysis products of trypsin that were used for internal calibration of spectra (mol wt: 842.51, 1045.56 and 2211.10, respectively). C. Parameters and results of SwissProt and Trembl database search.