Abstract
Normal, deprived (thymectomized, irradiated) and reconstituted (thymectomized, irradiated, thymus grafted) CBA mice were immunized with either a polyvalent or a mainly monovalent conjugate of the hapten 3-iodo-4-hydroxy-5-nitrophenylacetic acid and bovine serum albumin. The humoral antibody response to the hapten was determined by the phage inactivation technique.
The response of deprived mice was always significantly lower than the response of either normal or reconstituted animals. However, by increasing the dose of antigen in these mice it was possible to elicit some degree of responsiveness, but only in those groups which had received the polyvalent conjugate.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brownstone A., Mitchison N. A., Pitt-Rivers R. Chemical and serological studies with an iodine-containing synthetic immunological determinant 4-hydroxy-3-iodo-5-nitrophenylacetic acid (NIP) and related compounds. Immunology. 1966 May;10(5):465–479. [PMC free article] [PubMed] [Google Scholar]
- Claman H. N., Chaperon E. A., Triplett R. F. Thymus-marrow cell combinations. Synergism in antibody production. Proc Soc Exp Biol Med. 1966 Aug-Sep;122(4):1167–1171. doi: 10.3181/00379727-122-31353. [DOI] [PubMed] [Google Scholar]
- Davies A. J., Leuchars E., Wallis V., Koller P. C. The mitotic response of thymus-derived cells to antigenic stimulus. Transplantation. 1966 Jul;4(4):438–451. doi: 10.1097/00007890-196607000-00008. [DOI] [PubMed] [Google Scholar]
- Kontiainen S., Mäkelä O. Determination of 19S and 7S components in an anti-hapten antibody. Ann Med Exp Biol Fenn. 1967;45(4):472–476. [PubMed] [Google Scholar]
- MILLER J. F. Studies on mouse leukaemia. The role of the thymus in leukaemogenesis by cell-free leukaemic filtrates. Br J Cancer. 1960 Mar;14:93–98. doi: 10.1038/bjc.1960.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller J. F., Mitchell G. F. Thymus and antigen-reactive cells. Transplant Rev. 1969;1:3–42. doi: 10.1111/j.1600-065x.1969.tb00135.x. [DOI] [PubMed] [Google Scholar]
- Mäkelä O. Assay of anti-hapten antibody with the aid of hapten-coupled bacteriophage. Immunology. 1966 Jan;10(1):81–86. [PMC free article] [PubMed] [Google Scholar]
- Raff M. C. Role of thymus-derived lymphocytes in the secondary humoral immune response in mice. Nature. 1970 Jun 27;226(5252):1257–1258. doi: 10.1038/2261257a0. [DOI] [PubMed] [Google Scholar]
- TAYLOR R. B. IMMUNOLOGICAL COMPETENCE OF THYMUS CELLS AFTER TRANSFER TO THYMECTOMIZED RECIPIENTS. Nature. 1963 Aug 31;199:873–874. doi: 10.1038/199873a0. [DOI] [PubMed] [Google Scholar]
- Taylor R. B. Cellular cooperation in the antibody response of mice to two serum albumins: specific function of thymus cells. Transplant Rev. 1969;1:114–149. doi: 10.1111/j.1600-065x.1969.tb00138.x. [DOI] [PubMed] [Google Scholar]
- Taylor R. B., Wortis H. H. Thymus dependence of antibody response: variation with dose of antigen and class of antibody. Nature. 1968 Nov 30;220(5170):927–928. doi: 10.1038/220927a0. [DOI] [PubMed] [Google Scholar]