Table 1.
48-h mortality (%)
|
|||
---|---|---|---|
Enzyme concentrationb | Liquidc | Lyophilizedd | BSAe |
0x | 0 ± 0 | 0 ± 0 | 0 ± 0 |
1x | 0 ± 0 | 0 ± 0 | 0 ± 0 |
5x | 0 ± 0 | 0 ± 0 | 0 ± 0 |
10x | 5 ± 5 | 0 ± 0 | 0 ± 0 |
100x | 10 ± 12 | 0 ± 0 | 0 ± 0 |
500x | 100 ± 0 | 0 ± 0 | 0 ± 0 |
1,000x | 100 ± 0 | 10 ± 12 | 0 ± 0 |
Data are the average of four replicates with five neonates ± standard deviation.
lx = 2.5 × 10−3 U/ml in laboratory water.
Ammonium sulfate suspension esterase preparation (lot 102K7062; Sigma Chemical, St. Louis, MO, USA).
Lyophilized esterase preparation (lot 039H7005; Sigma Chemical).
BSA = bovine serum albumin.