Table 3.
Relative amount of actin based on LC1 (A) and α-actinin (B)
| Parameters | Control | Gelsolin | 2Ac | 3Ac | 4Ac | MAc |
|---|---|---|---|---|---|---|
| A | ||||||
| Actin/LC1 | 6.16 ± 0.56 | 1.26 ± 0.21 | 6.64 ± 2.20 | 6.10 ± 1.43 | 6.18 ± 1.17 | 6.92 ± 1.03 |
| *Relative amount of actin (%) | 100 | 20 ± 4 | 108 ± 37 | 99 ± 25 | 100 ± 21 | 112 ± 20 |
| N | 13 | 5 | 3 | 3 | 3 | 6 |
| B | ||||||
| Actin/α-actinin | 3.80 ± 0.35 | 1.10 ± 0.21 | 4.00 ± 0.47 | 3.06 ± 0.55 | 5.13 ± 0.36 | 3.96 ± 0.42 |
| *Relative amount of actin (%) | 100 | 29 ± 6 | 105 ± 16 | 81 ± 16 | 135 ± 16 | 104 ± 15 |
| N | 14 | 3 | 5 | 3 | 4 | 4 |
The intensity of α-actinin, actin, and LC1 bands was measured from SDS-PAGE, the actin intensity was normalized against LC1 intensity in A, and the actin intensity was normalized against α-actinin in B, and averaged. Means ±s.e.m. are shown.
*
The relative amount of actin was obtained by dividing the ratio by that of the control (6.16 ± 0.56 in A, 3.80 ± 0.35 in B) and the errors were propagated.