Table 1.

Hypoxia elicits varying responses on cytosolic Ca²⁺ in T lymphocytes (mixed CD3⁺ and CD3⁺ co-cultured with PBMCs) in five donors

	Resting cells
	Decrease (%)	No change (%)	Increase (%)	n	Transient (%)
Donor 1	22	70	8	74	6
Donor 2	38	44	17	81	8
Donor 3	11	75	14	44	4
Donor 4	75	21	4	91	3
Donor 5	70	26	4	23	13
Average	43	47	10	63	7
PHA pre-activated cells
Donor 1	96	0	4	48	30
Donor 3	29	40	31	78	12
Donor 4	47	43	10	147	15
Donor 5	60	26	14	100	17
Average	50	32	18	76	15

n, number of anti-CD3/CD28-stimulated cells (not including transient responders). Transient responders are reported as a percentage of all cells (stimulated and unstimulated). These cells did not sustain high Ca²⁺ levels when stimulated, but rapidly returned to baseline without experimental provocation. Hence, they were excluded from hypoxia analysis.