Figure 6. The influence of long exposures to hypotonic solutions under Cl⁻-free conditions.

Three muscles (A, B and C) were equilibrated with isotonic (225 mosmol l⁻¹) Cl⁻-free SO₄ ²⁻ Ringer solution, exposed to hypotonic (170 mosmol l⁻¹) Cl⁻-free Ringer solution (a) for different durations and finally returned to isotonic Cl⁻-free Ringer solution (b). Data points show mean relative volume, V_c (±s.e.m., n≥ 4 fibres in each case). Horizontal bars denote predicted cell volume, V_p, after each solution change.