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. 2006 May 2;103(19):7315–7320. doi: 10.1073/pnas.0510033103

Fig. 3.

Fig. 3.

GH signaling increases myotube size but does not affect myogenesis. (A) Myoblasts were induced to differentiate for 48 h in the presence of GH, and myotube diameter was measured and expressed as percentage change over control wild-type (+/+) cells. Histograms are means ± SEM of at least eight experiments from three independent cell cultures. ∗, P < 0.005, vs. +/+ control. Bright-field images of myotubes from +/+ and GHR−/− cells are shown. (B) GHR−/− myoblasts were transduced with GHR (GHRAdlox) or β-galAdlox adenoviruses at 30, 100, and 300 multiplicities of infection and induced to differentiate for 48 h. Myotube diameter was measured and is expressed as percentage change over +/+ cells transduced by β-galAdlox. Histograms are means ± SEM of four experiments on two independent cultures. ∗, P < 0.005, vs. GHR−/− transduced by β-galAdlox. Expression of GHR mRNA was determined by semiquantitative RT-PCR analysis. Gapdh expression was used as a control.