Figure 7.

Synthetic lethality between clb5 mutation and mutations in the PKC pathway. (A) The clb5Δ (JCY117), pkc1-8 (JC6-3a), slt2Δ (DL454), clb5Δ pkc1-8 (JCY161), and clb5Δ slt2Δ (JCY159) strains transformed with a control vector or a centromeric plasmid containing the CLB5, PKC1, or SLT2 gene were streaked onto YPD plates. Plates were incubated at 28° or 37° for 3 days. (B) Ten-fold serial dilutions from exponentially growing cultures of the clb5Δ (E143), swi4Δ (BY604), slt2Δ (DL454), and pkc1Δ (GPY115) strains transformed with a control vector and a centromeric plasmid containing the CLB5, SWI4, SLT2, or PKC1 gene, respectively, were spotted onto YPD medium with or without rapamycin (2–10 ng/ml) and incubated at 28° for 3 days. In the case of the pkc1Δ mutant, the growth medium was supplemented with 1 m sorbitol.