TABLE 3.
Suppression of git3Δ by cgs2-s1 and cgs2-2 in haploid and diploid strains
| β-Galactosidase activity
|
|||
|---|---|---|---|
| Strain | Genotype | Repressed | Derepressed |
| FWP72 | git3+cgs2+ | 3 ± 0 | 1369 ± 142 |
| RWP9 | git3Δ cgs2+ | 925 ± 10 | 1282 ± 10 |
| LWP167 | git3Δ cgs2-s1 | 27 ± 10 | 527 ± 56 |
| LWP161 | git3Δ cgs2-2 | 4 ± 0 | 52 ± 20 |
| LWP127 × LWP110 | git3+/git3+cgs2+/cgs2+ | 6 ± 1 | 1754 ± 32 |
| LWP156 × CHP934 | git3Δ/git3Δ cgs2+/cgs2+ | 513 ± 130 | 1573 ± 32 |
| LWP156 × CHP769 | git3Δ/git3Δ cgs2+/cgs2-s1 | 68 ± 15 | 970 ± 234 |
| LWP156 × LWP159 | git3Δ/git3Δ cgs2+/cgs2-2 | 4 ± 1 | 43 ± 22 |
β-Galactosidase activity was determined from three independent cultures as described in materials and methods. The average ±SE represents specific activity per milligram of soluble protein.