TABLE 5.
Synthetic defect in fbp1-lacZ derepression by cgs2-s1 and gpa2R176H
| β-Galactosidase activity
|
|||
|---|---|---|---|
| Strain | Genotype | Repressed | Derepressed |
| FWP72 | gpa2+cgs2+ | 3 ± 0 | 1369 ± 142 |
| CHP733 | gpa2+cgs2-s1 | 4 ± 0 | 263 ± 12 |
| RWP1 | gpa2R176Hcgs2+ | 2 ± 0 | 483 ± 5 |
| CHP732 | gpa2R176Hcgs2-s1 | 3 ± 0 | 12 ± 3 |
| CHP384 | gpa2+cgs2-2 | 3 ± 0 | 24 ± 2 |
Cells were grown in PM complete medium and β-galactosidase activity was determined from three independent cultures as described in materials and methods. The average ±SE represents specific activity per milligram of soluble protein.