TABLE 3.
Genes analyzed for their ability to suppress the temperature-sensitive phenotype of the tif51A-1 mutant
| Gene | Functional information | Suppression |
|---|---|---|
| TIF51A | Putative translation initiation factor eIF5A | + |
| WSC1 | Sensor for cell wall integrity signaling, plays a role in activation of the Pkc1-MAPK pathway; Wsc family | + |
| WSC2 | Putative sensor for cell wall integrity signaling; Wsc family | + |
| WSC3 | Putative sensor for cell wall integrity signaling; Wsc family | + |
| MID2 | Sensor for cell wall integrity signaling, plays a role in activation of the Pkc1-MAPK pathway; Mtl1 homolog | + |
| MTL1 | Protein that acts in signal transduction of cell wall stress, plays a role in regulation of cell integrity pathway; Mid2 homolog | + |
| ROM2 | GDP/GTP exchange factor (GEF) for Rho1 and Rho2 | + |
| RHO2 | GTP-binding protein, member of the rho subfamily of ras-like proteins | + |
| PKC1 | Protein kinase C, regulates MAP kinase cascade involved in regulating cell wall metabolism (cell integrity pathway) | + |
| BCK1-20a | MAP kinase kinase kinase component of cell integrity pathway | − |
| MKK1 | MAP kinase kinase component of cell integrity pathway | − |
| MPK1 | MAP kinase component of cell integrity pathway | − |
| ZDS1 | Protein with effects on cell polarity and transcriptional silencing, homolog of Zds2 | + |
| GIC1 | Effector of Cdc42, important for bud emergence; Gic2 homolog | + |
| PCL1 | G1/S-specific Pho85 cyclin | + |
| PCL2 | G1/S-specific Pho85 cyclin | − |
| CLN2 | G1/S-specific Cdc28 cyclin | − |
| GIC2 | Effector of Cdc42, important for bud emergence; Gic1 homolog | − |
| CDC42 | Rho-type GTPase involved in bud site assembly and cell polarity | − |
| CLA4 | PAK kinase required for cytokinesis, effector of Cdc42 | − |
| STE20 | PAK kinase of the pheromone pathway; also regulates polarized growth, effector of Cdc42 | − |
| CDC24 | GEF for Cdc42, involved in bud emergence, bud site selection, and growth of mating projection | − |
| BUD1 | GTP-binding protein of the ras superfamily involved in bud site selection | − |
| BEM1 | Scaffold protein for complexes involving cell polarity establishment and morphogenesis factors such as Cdc24 and Bud1 | + |
| BEM2 | Rho-type GTPase-activating protein (GAP) for Rho1 | − |
| BNI1 | Formin protein involved in cytoskeletal polarization and cytokinesis | + |
| ZDS2 | Protein with effects on cell polarity and transcriptional silencing, homolog of Zds1 | + |
| RGA1 | Rho-type GAP for Cdc42 | − |
| BEM3 | Rho-type GAP for Cdc42 | − |
| SSD1 | mRNA binding protein; plays a role in maintenance of cellular integrity | + |
a Encodes a constitutively activated version of Bck1 (Lee and Levin 1992).