TABLE 2.
Principal CH parameters of 5-day-old male and female flies homozygous for various desat11573-1 alleles and the size of the transposon inserted into desat1
| Male
|
Female
|
Transposon length (kb)
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| DI | ΣDesat (%) | ΣLin (%) | SumCH | DI | ΣDesat (%) | ΣLin (%) | SumCH | |||
| Wild-type: | Cs | +0.566 (0.041) | 62.7 (2.2) | 17.2 (1.4) | 982 (81) | +0.687 (0.017) | 61.0 (1.0) | 11.3 (0.6) | 1788 (181) | |
| Mutant: | 1573 | −0.811 (0.010) | 7.1 (0.4) | 68.5 (0.8) | 3033 (165) | −0.844 (0.010) | 5.4 (0.3) | 64.3 (1.1) | 2681 (89) | 11.3 |
| Excision-mutant | ||||||||||
| E1 | −0.894 (0.010) | 3.9 (0.4) | 70.1 (0.6) | 2921 (73) | −0.828 (0.020) | 5.4 (0.6) | 57.5 (1.2) | 2023 (120) | 6.2 | |
| J2 | −0.891 (0.011) | 4.0 (0.4) | 68.9 (1.0) | 2366 (244) | −0.763 (0.013) | 7.8 (0.4) | 58.4 (0.9) | 2282 (70) | 8.7 | |
| F3 | −0.880 (0.008) | 4.2 (0.2) | 66.6 (1.4) | 2471 (108) | −0.866 (0.003) | 4.5 (0.1) | 63.0 (0.4) | 1804 (251) | 9.6 | |
| P3 | −0.873 (0.029) | 4.7 (1.1) | 69.7 (1.7) | 2481 (59) | −0.743 (0.012) | 9.1 (0.5) | 62.0 (0.9) | 2029 (57) | 10.6 | |
| P′2 | −0.872 (0.008) | 4.7 (0.3) | 69.1 (0.4) | 1760 (219) | −0.752 (0.011) | 8.4 (0.6) | 58.8 (1.0) | 2219 (62) | 10.3 | |
| C1 | −0.863 (0.013) | 5.2 (0.5) | 70.7 (0.7) | 2897 (176) | −0.872 (0.005) | 4.6 (0.2) | 67.3 (0.2) | 2086 (190) | 10.6 | |
| C6 | −0.862 (0.025) | 5.1 (1.1) | 68.5 (0.6) | 2695 (194) | −0.660 (0.060) | 11.6 (2.0) | 56.6 (2.6) | 2459 (37) | 8.1 | |
| C2 | −0.855 (0.012) | 5.8 (0.4) | 73.7 (1.0) | 2713 (248) | −0.882 (0.015) | 4.2 (0.5) | 67.8 (1.0) | 1880 (36) | 10.6 | |
| C3 | −0.849 (0.018) | 5.9 (0.6) | 72.0 (1.4) | 2524 (50) | −0.696 (0.025) | 10.3 (0.8) | 57.5 (0.8) | 2090 (147) | 8.1 | |
| A4 | −0.844 (0.015) | 6.1 (0.5) | 72.6 (1.3) | 2726 (170) | −0.821 (0.014) | 5.9 (0.4) | 60.4 (0.8) | 2136 (27) | 11.0 | |
| D1 | −0.824 (0.010) | 7.0 (0.3) | 72.4 (1.2) | 1825 (174) | −0.823 (0.014) | 6.3 (0.6) | 64.9 (0.4) | 2296 (105) | 9.7 | |
| C5 | −0.817 (0.008) | 7.0 (0.4) | 69.2 (2.6) | 2798 (245) | −0.542 (0.040) | 15.9 (1.2) | 53.7 (2.1) | 2551 (119) | 6.9 | |
| P2 | −0.779 (0.008) | 8.4 (0.3) | 68.2 (0.5) | 3051 (202) | −0.819 (0.014) | 6.3 (0.5) | 63.5 (0.9) | 2376 (210) | 7.7 | |
| Q′1 | −0.711 (0.039) | 10.9 (1.3) | 64.5 (3.2) | 2266 (240) | −0.605 (0.006) | 13.1 (0.6) | 52.0 (1.6) | 2122 (135) | 6.4 | |
| Excision-intermediate | ||||||||||
| H6 | +0.171 (0.030) | 41.8 (1.3) | 29.5 (1.1) | 1278 (101) | −0.112 (0.055) | 32.8 (2.0) | 41.1 (2.1) | 1873 (83) | 8.6 | |
| L6 | +0.296 (0.026) | 51.1 (1.1) | 27.8 (1.2) | 1508 (98) | +0.053 (0.045) | 36.8 (1.6) | 33.2 (1.6) | 1969 (64) | 5.1 | |
| F7 | +0.391 (0.070) | 48.5 (2.7) | 21.1 (2.4) | 1467 (67) | −0.389 (0.087) | 22.5 (3.2) | 52.0 (3.7) | 1561 (92) | 0.1 | |
| F4 | +0.395 (0.032) | 55.2 (1.3) | 24.0 (1.4) | 1327 (59) | −0.089 (0.051) | 33.2 (1.6) | 40.2 (2.3) | 1884 (73) | 0.1 | |
| M3 | +0.399 (0.097) | 56.1 (3.0) | 24.0 (2.7) | 1452 (97) | −0.070 (0.038) | 33.4 (1.1) | 38.7 (1.8) | 1636 (47) | 6.8 | |
| L2 | +0.429 (0.018) | 52.4 (1.1) | 20.9 (0.6) | 1373 (52) | +0.073 (0.041) | 36.0 (1.3) | 31.3 (1.5) | 1496 (46) | 5.8 | |
| F6 | +0.446 (0.066) | 59.0 (2.6) | 22.9 (2.8) | 1690 (93) | −0.048 (0.070) | 32.8 (2.2) | 36.7 (2.7) | 1925 (113) | 0.1 | |
| F2 | +0.452 (0.025) | 56.1 (1.3) | 21.1 (0.9) | 1434 (76) | +0.075 (0.048) | 37.1 (1.7) | 32.0 (1.9) | 1812 (127) | 0.1 | |
| Excision-rescue | ||||||||||
| N2 | +0.594 (0.025) | 62.1 (0.9) | 15.9 (1.1) | 1284 (63) | +0.475 (0.047) | 50.5 (1.6) | 18.0 (1.7) | 1604 (67) | 0 | |
| L3 | +0.644 (0.026) | 62.9 (1.0) | 13.7 (1.1) | 1070 (237) | +0.457 (0.022) | 48.6 (1.0) | 18.1 (0.8) | 1475 (38) | 0 | |
| A′2 | +0.676 (0.023) | 61.8 (1.9) | 11.9 (0.8) | 1141 (201) | +0.539 (0.016) | 52.7 (1.0) | 15.8 (0.4) | 1437 (57) | 0 | |
All CH data are mean (±SEM) for N = 10 flies. Excision alleles were classified into three categories according to their DI (see materials and methods). Within each category, alleles were ranked from top to bottom according to male DIs. In mutant alleles (Excision-mutant) DIs were different from that of the control (Cs) and similar to that of mutant 1573-1. DIs of rescued alleles (Excision-rescue) were similar to that of Cs and different from that of 1573-1. DIs of intermediate alleles (Excision-intermediate) were different from the DIs of both Cs and 1573-1, either for both sexes (H6) or for only females (L6–F2). ΣDesat (%) corresponds to the total percentage of all unsaturated CHs relative to the sum of detected CHs (SumCH, given in nanograms); ΣLin (%) corresponds to the total percentage of all saturated linear CHs. The transposon length (shown at the right) indicates the estimated size (in kilobases) of the fragment of the transposon that remains inserted in each allele. This estimation corresponds to the pooled size of three sequences detected by Southern blots.