TABLE 3.

Comparison of the quantity of five desat1 transcripts in 24-hr-old male and female flies of various genotypes

		Female		Male
Transcript	Genotype	a.u.	%	a.u.	%	Quantity
Canton-S	275 (41)	5.58	117 (5)	2.63	0.001
RA	N2	232 (27)	2.83	133 (10)	1.78	0.001
1573	−22 (5)	0.17	−59 (11)	0.18	0.017
0.001	0.005
Canton-S	4587 (82)	93.20	4292 (579)	96.36	0.248
RC	N2	8085 (637)	96.04	7289 (1226)	97.55	1
1573	2.4 (0.2)	8.87	0.7 (0.6)	7.44	0.021
0.007	0.012
Canton-S	8 (2)	0.16	12 (3)	0.28	0.248
RE	N2	9 (1)	0.11	16 (1)	0.22	0.021
1573	0 (0)	0	0 (0)	0	1
0.021	0.020
Canton-S	51 (7)	1.04	32 (4)	0.71	0.043
RB	N2	85 (7)	1.01	33 (1)	0.44	0.021
1573	23 (2)	87.19	7.8 (0.8)	81.88	0.021
0.007	0.023
Canton-S	1	0.02	1	0.02
RD	N2	1	0.01	1	0.01
	1573	1	3.77	1	10.51

The quantity of each transcript was evaluated in arbitrary units (a.u.) with the amount of “RD” transcript chosen as the reference unit. Data shown correspond to the mean (±SEM) of a duplicated quantification carried out with two independent extractions (N = 2 + 2). Nonparametrical statistics allow us to compare the genotypes (with a Kruskal-Wallis test) and the sexes (with a Mann-Whitney test, except for “RD,” which was constant). The percentage indicates the representativeness of each transcript relative to the total amount of detected transcripts in each sex and genotype. The negative value obtained in 1573-1 flies indicates that the quantity of “RA” was a fraction (1/22 in females, 1/59 in males) of 1 a.u