Figure 3.

Mapping of the free 5′ ends for determining the position of the O_N of insect mtDNA. (A) The nascent DNA strands of the minor coding strand were amplified, and LMPCR product was resolved in a 5% acrylamide/6 m urea gel (sample lane) with the sequence ladders, which were applied in parallel (Lanes A, C, G, and T). (B) Location of the free 5′ ends marking the O_N. The sequences of the template strands are the portion that is next to the 12S rRNA gene for B. mori and the middle portion for T. castaneum and L. migratoria in the A + T-rich region. The sequences shown for B. mori and T. castaneum are from Yukuhiro et al. (2002) and Friedrich and Muqim (2003), respectively. See Figure 2 for an explanation of symbols. (C) Possible stem-loop structures formed immediately upstream of the O_N of L. migratoria mtDNA. The nucleotide sequence of L. migratoria, which potentially forms the stem-loop structure, is underlined in B. The possible stem-loop structures of S. gregaria and C. parallelus are from Zhang et al. (1995).