Figure 1.
PTHrP-increased expression of Col2a1 RNA. (A) RCS cells were cultured for 24 h before 10−7 M PTHrP was added; the cells were cultured further for different intervals as indicated. Cells were then collected, and total RNA was extracted and hybridized with a Col2a1 probe. The hybridization membrane was rehybridized with Sox9 and glyceraldehyde-3-phosphate dehydrogenase probes. (B) 10T1/2 cells were transfected with an empty vector (lane 1) or a PTH/PTHrP receptor expression plasmid (lanes 2–6) for 24 h. Different concentrations of PTHrP were then added as indicated, and the cells were cultured for another 4 h. Northern hybridization was performed as in A.