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. 2000 Dec 19;98(1):160–165. doi: 10.1073/pnas.011393998

Figure 3.

Figure 3

PTHrP-increased phosphorylation of SOX9 at S181. (A) COS7 cells were transfected with a SOX9 expression plasmid (100 ng) (lane 1) or cotransfected with SOX9 (100 ng) and PTH/PTHrP receptor (200 ng) expression plasmids (lanes 2–6). PTHrP was (lanes 1 and 3–6) or was not (lane 2) added as indicated. Cell lysates were fractionated by using SDS/PAGE and blotted, and the blots were incubated with Sox9.P and Sox9 antibodies. (B) COS7 cells were cotransfected with SOX9 (100 ng) and PTH/PTHrP receptor (200 ng) expression plasmids. No PTHrP (lane 1) or 10−7 M PTHrP (lanes 2–6) with H89 (lanes 3 and 4) or OA (lanes 5 and 6) was added. Cells were then cultured for an additional 4 h before lysis.