Figure 3.

Amino acid sequences of the medaka SLR and GHR. (A) Amino acid alignment of the SLRs and GHRs of medaka, fugu, and masu salmon. Those of fugu are putative (see text). Major residues among each group are in red. Asterisks indicate that the major residues for SLRs and GHRs are identical. Boxes indicate residues that contain exon-intron boundaries in their codons or the first residues in each exon. The conserved cysteines (six disulfide linked and one unpaired), FGXFS motif, box 1, box 2, and tyrosines (see text) are highlighted in yellow. (B) A phylogenic tree drawn by the Neighbor program in the PHYLIP package (http://evolution.genetics.washington.edu/phylip.html) using entire sequences of the SLRs and GHRs. All nodes are supported by bootstrap values of 100, demonstrating an orthologous relationship among the SLRs and GHRs of these fish (see also Figure 7).