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. 2006 Jan;172(1):99–112. doi: 10.1534/genetics.105.050427

Figure 4.

Figure 4.

SUM1-1 repression was promoter specific and unstable. (A) MATα sir2Δ sum1Δ strains in which transcription of the MATa1 coding region was driven by the MATa1 promoter (ROY2863) or by the URA3 promoter (ROY3723) at the HMR locus were transformed with vector alone (pRS425), pSUM1 (pRO709), pSUM1-1 (pRO711), or pSIR2 (pRO46). Transformants were grown selectively and fivefold serial dilutions were spotted onto YMD plates lacking leucine to monitor growth or onto YMD plates with a MATa tester lawn (MATa his4) to monitor for expression of the MATa1 gene using a mating assay. (B) MATα sir2Δ sum1Δ strains with the URA3 coding region under the control of the MATa1 promoter (ROY3306) or the URA3 promoter (ROY3769) were transformed with vector alone (pRS425), pSUM1 (pRO709), pSUM1-1 (pRO711), or pSIR2 (pRO46). The transformants were grown selectively and fivefold serial dilutions were spotted onto YMD plates without leucine as controls for growth or onto YMD plates containing 5-FOA (5-FOA–LEU) to monitor for stable repression of URA3.