Figure 5.

Sum1-1p-mediated repression was not stably inherited. Three different MATα strains with the URA3 coding region under the control of the MATa1 promoter were generated. ROY4043 was SIR2 SUM1 while ROY4042 was sir2Δ SUM1 and ROY3364 was sir2Δ SUM1-1. The strains were grown in liquid YPD medium, and then placed onto YMD plates with 5-FOA. Single cells were micromanipulated on the plates and the plates were photographed to monitor growth and division of the cells. At 24 hr postmicromanipulation, the number of cells in each microcolony were counted. Approximately 200 microcolonies were counted for each strain and the data were plotted as a percentage of the total.