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Sum1-1p-mediated repression was not stably maintained. MATa hmlΔ HMRα strains with SIR2 SUM1 or sir2Δ SUM1-1 alleles were initially grown in liquid YPAD medium. The cells were arrested with α-factor for 3 hr in liquid medium and then streaked onto YPAD plates. Single cells were micromanipulated on the regions of the plate containing or lacking α-factor and the micromanipulated cells were photographed every 3 hr for growth and cell division.
