Figure 7.

SUM1-1-repressed genes within a large domain. MATα SUM1-1 sir2Δ strains (ROY 3322, ROY 3324, ROY 3259, and ROY4029) containing the MATa1 reporter gene integrated at different locations were grown in YPD liquid medium and 3 μl of fivefold serial dilutions were spotted onto mating lawns to assay for the expression of the MATa1 genes. A schematic shows the sites of insertion and orientation of the MATa1 reporter gene integrated within the HMR locus. “BarrierΔ” refers to a replacement of the HMR barrier sequences with pUC DNA.