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Indirect end-labeling analyses of the HMR domain. Permeabilized spheroplasts from wild type (JRY4013), sir2Δ SUM1 (JRY4563), and sir2Δ SUM1-1 (ROY1924) strains were digested with micrococcal nuclease. After deproteinization and restriction enzyme digestion, the DNA was resolved on an agarose gel, blotted, and hybridized with a probe specific to HMR.
