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. 2006 Jan;172(1):99–112. doi: 10.1534/genetics.105.050427

TABLE 1.

Yeast strains used in this study

Strain Genotype Source
MATahis4 J. Rine
MATα his4 J. Rine
JRY19a MATaura3 leu2 trp1 his4 J. Rine
ROY3790 MATα HMR∷TRP1 SIR2 SUM1
ROY3792 MATα HMR∷TRP1 sir2Δ∷HIS3 SUM1
ROY3839 MATα HMR∷TRP1 sir2Δ∷HIS3 SUM1-1
ROY3774 MATα HMR∷ADE2 SIR2 SUM1
ROY3770 MATα HMR∷ADE2 sir2Δ∷HIS3 SUM1
ROY3818 MATaHMR∷ADE2 sir2Δ∷TRP1 SUM1-1
ROY3787 MATα HMR∷URA3 ppr1Δ∷kanMX SUM1 SIR2
ROY3797 MATaHMR∷URA3 ppr1Δ∷kanMX SUM1 sir2Δ∷ TRP1
ROY2677 MATα HMR∷URA3 ppr1Δ∷kanMX SUM1-1 sir2Δ∷TRP1
ROY4027 MATα HMR∷URA3 ppr1Δ∷kanMX SUM1 SIR2 ura3Δ∷HIS3
ROY4028 MATaHMR∷URA3 ppr1Δ∷kanMX SUM1 sir2Δ∷ TRP1 ura3Δ∷HIS3
ROY4026 MATα HMR∷URA3 ppr1Δ∷kanMX SUM1-1 sir2Δ∷ TRP1 ura3Δ∷HIS3
JRY4013 MATα HMRaSUM1 SIR2 J. Rine
JRY4563 MATα HMRaSUM1 sir2Δ∷ TRP1 J. Rine
ROY1924 MATα HMRaSUM1-1 sir2Δ∷ TRP1
ROY3786 MATaHMLα hmrΔ∷URA3 SUM1-1 sir2Δ∷HIS3
ROY3753 MATα HMLahmrΔ∷URA3 SUM1-1 sir2Δ∷HIS3
ROY3754 MATα HMLahmrΔ∷URA3 SUM1 SIR2
ROY3755 MATα HMLahmrΔ∷URA3 SUM1 sir2Δ∷HIS3
ROY3785 MATα hmlΔ∷TRP1 HMRaSUM1-1 sir2Δ∷HIS3
ROY3725 MATahmlΔ∷TRP1 HMRα SUM1 sir2Δ∷HIS3
ROY3726 MATahmlΔ∷TRP1 HMRα SUM1 SIR2
ROY3743 MATahmlΔ∷TRP1 HMRα SUM1-1 sir2Δ∷HIS3
ROY3861 MATahmlΔ∷TRP1 hmrΔ∷HMLα SUM1 SIR2
ROY3859 MATahmlΔ∷TRP1 hmrΔ∷HMLα SUM1 sir2Δ∷HIS3
ROY3863 MATahmlΔ∷TRP1 hmrΔ∷HMLα SUM1-1 sir2Δ∷HIS3
ROY4030 MATahmlΔ∷TRP1 hmrΔ∷[HMR-E-α-HML-I] SUM1 sir2Δ∷HIS3
ROY4031 MATahmlΔ∷TRP1 hmrΔ∷[HMR-E-α-HML-I] SUM1 SIR2
ROY4032 MATahmlΔ∷TRP1 hmrΔ∷[HMR-E-α-HML-I] SUM1-1 sir2Δ∷HIS3
ROY4053 MATahmlΔ∷TRP1 hmrΔ∷[HML-E-α-HMR-I] SIR2 SUM1
ROY4045 MATahmlΔ∷TRP1 hmrΔ∷[HML-E-α-HMR-I] sir2Δ∷HIS3 SUM1
ROY4048 MATahmlΔ∷TRP1 hmrΔ∷[HML-E-α-HMR-I] sir2Δ∷HIS3 SUM1-1
ROY2863 MATα sum1Δ∷kanMX sir2Δ∷TRP1 HMR-[E+a2∷prma1-a1cds+a1Δp+I]
ROY3306 MATα sum1Δ∷kanMX sir2Δ∷TRP1 HMR-[E+a2∷prma1-URA3cds+a1Δp+I]
ROY3723 MATα sum1Δ∷kanMX sir2Δ∷TRP1 HMR-[E+a2∷prmURA3-a1cds+a1Δp+I] ppr1Δ∷kanMX
ROY3769 MATα sum1Δ∷kanMX sir2Δ∷TRP1 HMR-[E+a2∷prmURA3-URA3cds+a1Δp+I] ppr1Δ∷kanMX
ROY3322 MATα sir2Δ∷TRP1 SUM1-1 HMRa1Δp∷a1
ROY3324 MATα sir2Δ∷TRP1 SUM1-1 HMRa1Δp - a1- tRNA
ROY3259 MATα sir2Δ∷TRP1 SUM1-1 HMRa1Δp tRNA barrier - a1
ROY4029 MATα sir2Δ∷TRP1 SUM1-1 HMRa1Δp tRNA barrierΔ - a1
ROY4043 MATα HMR-prma1+URA3cds-hmraΔp SIR2 SUM1
ROY4042 MATα HMR-prma1+URA3cds-hmraΔp sir2Δ∷TRP1 SUM1
ROY3364 MATα HMR-prma1+URA3cds-hmraΔp sir2Δ∷TRP1 SUM1-1
ROY2666 MATα HMR sir2Δ∷HIS3 sum1Δ∷kanMX
ROY4038 MATα HMRss(1xGAL4-RAP1-ABF1)+I sir2Δ∷HIS3 sum1Δ∷kanMX
ROY4039 MATα HMRss(1xGAL4-RAP1-ABF1) Δ I sir2Δ∷HIS3 sum1Δ∷kanMX
ROY4040 MATα HMRss(3xGAL4-RAP1-ABF1) Δ I sir2Δ∷HIS3 sum1Δ∷kanMX
ROY4041 MATα HMRss(5xGAL4-RAP1-ABF1) Δ I sir2Δ∷HIS3 sum1Δ∷kanMX