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Single cell analyses for repression of HMRα
|
MATahmlΔ HMRα
|
||||
|---|---|---|---|---|
|
SIR2SUM1
|
sir2Δ SUM1-1
|
|||
| α-Factor | + | − | + | − |
| Budding | 0 | 88 | 55 | 78 |
| Shmoo | 100 | 12 | 45 | 22 |
At 1 hr after micromanipulation, the cells in each microcolony were monitored for the presence of shmooed undivided single cells vs. cells that had divided multiple times to form microcolonies with greater than two cells per colony. The strains monitored were MATa hmlΔ HMRα with SIR2 SUM1 or sir2Δ SUM1-1 alleles. Numbers are percentages.
