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. 2006 Jan;172(1):343–353. doi: 10.1534/genetics.105.049650

Figure 4.

Figure 4.

Subcellular localization of the TW and RT proteins. (A–C) Immunofluorescent staining for HA-tagged TW protein expressed in the third larva instar salivary gland cells of PDI∷GFP transgenic flies (Bobinnec et al. 2003). (A) TW (red, Cy5); (B) PDI-GFP (green); (C) overlay of the red A and green B channels. The staining reveals TW localization in the ER compartment. (D–F) Double-immunofluorescent staining for HA-tagged TW and LVA proteins expressed in the Drosophila S2 cell culture. (D) LVA (red, Cy3); (E) TW (green, FITC); (F) overlay of the red D and green E channels. The staining indicates TW exclusion from the Golgi compartment. (G–I) Double-immunofluorescent staining for TW (HA-tagged) and RT (MYC-tagged) coexpressed in Drosophila culture cells. (G) TW (red, Cy3); (H) RT (green, FITC); (I) overlay of the red (G) and green (H) channels. The double immunostaining shows colocalization of TW and RT within Drosophila cells. Circular staining around nuclei of S2 cells represents perinuclear ER (Okajima et al. 2005). Bars, 20 μm in C and 6 μm in F and I.