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. 2006 Jan;172(1):41–51. doi: 10.1534/genetics.105.045815

Figure 2.

Figure 2.

Viablity of sti1 mutants in an sti1ydj1 strain. (A) Strain JJ609 (sti1ydj1/pRS316–YDJ1) was transformed with plasmids containing WT STI1 or sti1 mutants. After overnight incubation at 30°, equal amounts of cells were plated onto selective media (C) to maintain WT YDJ1 or media containing 5-FOA to counterselect for YDJ1 (FOA). Plates were incubated at 25° for 5 days. (B) Genetic screen to isolate sti1 mutants that fail to support viability of a sti1ydj1 strain. A plasmid DNA library containing random mutations in STI1 was transformed into strain JJ609. Transformants were patched onto media to maintain YDJ1 or onto media containing 5-FOA to counterselect for YDJ1. Colonies that failed to grow in the presence of 5-FOA were selected for further study.