Figure 3.

Sti1 mutants obtained in genetic screen. (A) Mutant sti1 plasmids obtained in the genetic screen were transformed into strain JJ623 (sti1). Equal amounts of lysates from sti1 cells expressing WT STI1 or indicated sti1 mutants were separated by SDS–PAGE (10% acrylamide) and immunoblotted with Sti1-specific antibodies. (B) Growth of serial dilutions of the same strains after a 2-day incubation at 37° on selective medium.