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Pooled sample mapping to detect recombinants within the cluster. Pools were constructed with EcoRI-digested DNA from eight F2 homozygous recessive segregants. DNA gel blots of these pools were probed with a P. vulgaris TIR probe. All 483 homozygous recessives from an F2 population of 3053 plants were screened with this procedure. No recombinants were detected.
