Figure 9.

RNA gel blot analysis. The primary leaves of 8-day-old seedlings of Calima (C) and Jamapa (J) were inoculated with (a) sterile water; (b) NL-1, a strain of BCMV; and (c) NL-3, a BCMNV strain. Leaf samples were harvested 3 days post-inoculation. Each lane contained 2 μg of poly(A)⁺ RNA. Agarose gels were stained with SYBR-gold to show approximately uniform loading of samples. Northern blots were hybridized with P. vulgaris clones of TIR and WRKY sequences and pMB123, a mung bean clone of the CAB-binding protein.