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. 2006 Feb;172(2):1263–1275. doi: 10.1534/genetics.105.051219

Figure 2.

Figure 2.

Tuber developmental stages and patatin RNA gel blot analysis. (A) The six tuber developmental stages sampled in this study were based on tuber length (see materials and methods). Examples of sample developmental stages are shown. Stage 1, Stolon; stage 2, 1–5 mm; stage 3, 6–10 mm; stage 4, 11–15 mm; stage 5, 16–25 mm; stage 6, 26–35 mm. The diameter of the coin is 24 mm. Mature tuber is shown on the right as a developmental reference in the growth chamber environment, but was not sampled in this study. (B) Patatin gene expression level increases throughout tuber development. The patatin transcripts at the different tuber developmental stages were detected by RNA gel blot analysis using a DNA probe from patatin cDNA pGM01. (C) Equal loading of total RNA was confirmed by gel staining for rRNA with ethidium bromide (data not shown) and by reprobing with a DNA probe from ubiquitin EST STMGC93.