. 2006 Feb;172(2):1277–1285. doi: 10.1534/genetics.105.049908

TABLE 1.

Detection patterns of barley-specific transcripts in Betzes, Chinese Spring, and Chinese Spring–Betzes chromosome addition lines harboring barley chromosome 2(2H), 3(3H), 4(4H), 7(5H), 6(6H), or 1(7H)

Genetic stock								No. of barley transcripts	No. of single-copy transcripts^a	No. of multiple-copy transcripts^a
Betzes	CS	2H	3H	4H	5H	6H	7H	No. of barley transcripts	No. of single-copy transcripts^a	No. of multiple-copy transcripts^a
+	−	+	365	344	21
+	−	+	271	243	28
+	−	+	265	240	25
+	−	+	323	301	22
+	−	+	194	187	7
+	−						+	369	342	27

Transcript accumulation patterns among Betzes, CS, and the wheat–barley disomic chromosome addition lines were determined on the basis of t-tests (P < 0.001) of transcript signals normalized by robust multichip analysis (RMA) followed by presence (+) or absence (−) test (detection P < 0.001 using microarray analysis suite 5.0) of 22,792 probe sets on the Barley1 Affymetrix GeneChip. See Figure 1 for details on the work flow. CS, Chinese Spring; 2H, 3H, 4H, 5H, 6H, and 7H are the wheat–barley chromosome addition lines carrying barley chromosomes 2(2H), 3(3H), 4(4H), 7(5H), 6(6H), and 1(7H), respectively.

Determination of single- or multiple-copy transcripts was based on specificity of a given transcript to each barley chromosome added to wheat.